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目的利用现代生物技术方法生物合成红景天苷,探索提高红景天苷含量的生物转化最佳条件,为工业化大生产提供科学依据。方法以转基因西洋参冠瘿组织为培养体系,生物转化底物酪醇;利用HPLC对转化产物红景天苷进行含量测定;考察不同培养条件对西洋参冠瘿组织生长量及其转化产物红景天苷的影响。结果在摇床培养中西洋参冠瘿组织培养基为“MS+蔗糖”中红景天苷转化率达到49.50%;“1/2MS+蔗糖”培养基的转化率为49.98%;“MS+蔗糖+0.5 mg.L-1VB6”培养基的转化率达55.54%;此外,还利用生物反应器对西洋参冠瘿组织生物转化红景天苷进行了初步研究,转化率为33.42%。结论本实验通过考察不同培养条件对西洋参冠瘿组织生物合成红景天苷的影响,提高了红景天苷的转化率,并在摇瓶培养的基础上,初步实现了生物反应器对西洋参冠瘿组织生物合成红景天苷的生产,从而为该产品的工业化生产提供科学资料。
OBJECTIVE: To synthesize salidroside by modern biotechnological methods and to explore the optimal biotransformation conditions for enhancing salidroside content, providing a scientific basis for industrialized large-scale production. Methods Transgenic American Ginseng Taraxacum cuspidate tissue culture system was used to transform the substrate tyrosol. HPLC was used to determine the content of salidroside. The effects of different culture conditions on the growth of crown ginseng tissue and the content of salidroside Impact. Results The conversion rate of salidroside in the “MS + sucrose” was 49.50% in the culture medium of crown ginseng, and the conversion rate of “1/2 MS + sucrose” was 49.98% The conversion rate of sucrose +0.5 mg.L-1VB6 "culture medium was 55.54%. In addition, biotransformation of salidroside in the crown gynogenetic tissue of American ginseng with bioreactor was also studied. The conversion rate was 33.42%. Conclusion In this experiment, we investigated the effects of different culture conditions on the biosynthesis of salidroside and the conversion rate of salidroside. Based on the shake flask culture, the effects of bioreactor on the growth of American ginseng root瘿 tissue biosynthesis salidroside production, so as to provide scientific information for the industrial production of the product.