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目的:探讨美托洛尔是否影响红细胞血红蛋白的携氧能力。方法:采集正常人红细胞,悬浮于不同pH值(pH7.4、pH5.0)等渗PBS缓冲液,分别用肾上腺素、美托洛尔、美托洛尔+肾上腺素预处理红细胞,在拉曼光谱点扫描分析检测单个活态红细胞内血红蛋白特征光谱变化。结果:在pH5.0时,氧化血红蛋白特征峰峰强在PBS、美托洛尔的作用下明显降低;在肾上腺素的作用下显著升高;而经美托洛尔+肾上腺素预处理后,肾上腺素的这一作用不明显。结论:美托洛尔能减轻肾上腺素在酸性环境中增加红细胞血红蛋白的氧合力的作用,增加氧从血红蛋白的解离,有利于缺氧组织的供氧。
Objective: To investigate whether metoprolol affects the oxygen carrying capacity of erythrocyte hemoglobin. Methods: Normal human erythrocytes were collected and suspended in isotonic PBS buffer at different pH values (pH7.4, pH5.0). Erythrocytes were pretreated with epinephrine, metoprolol, metoprolol and epinephrine respectively. Mannographic point-scan analysis examines the characteristic spectral changes of hemoglobin within a single live erythrocyte. Results: At pH 5.0, the characteristic peak of oxidized hemoglobin was significantly lower under the action of PBS and metoprolol, significantly higher under the action of epinephrine. After metoprolol and epinephrine pretreatment, This effect of epinephrine is not obvious. CONCLUSION: Metoprolol can reduce the effect of epinephrine on the oxygenation of erythrocyte hemoglobin in acidic environment and increase the dissociation of oxygen from hemoglobin, which is in favor of supplying oxygen to hypoxic tissues.