论文部分内容阅读
利用 Yop1- Mc Ab- RIHA(反向间接血球凝集试验 )检测全国 17个生态型 10 5株鼠疫耶氏菌 (Yersinia pestis)强毒株和 4株弱毒标准株、6株不同血清型的假结核耶氏菌、4株小肠结肠炎耶氏菌以及 3株其它肠道菌。结果显示 ,假结核和小肠结肠炎耶氏菌呈现较高滴度的阳性反应 (滴度 1∶ 2 7～ 1∶ 2 1 0 ) ,其余均为阴性 ,提示 :(1) Yop1蛋白为耶尔森氏菌所特有 ;(2 )鼠疫耶氏菌中突变的 yop A基因发生回复突变的可能性不大 ,进一步的结论将在扩大检测后作出 ;(3)据此 ,有理由认为 Yop1蛋白单克隆抗体及其检测技术在鼠疫与假结核耶氏菌及小肠结肠炎耶氏菌 O∶ 9血清型与布鲁氏菌的鉴别诊断上有良好的应用前景。
Yop1-Mc Ab-RIHA (reverse indirect hemagglutination test) was used to detect 105 strains of Yersinia pestis and 4 attenuated standard strains of 17 ecotypes in China. Six pseudotuberculosis strains of different serotypes Yersinia, four strains of Enterocolitis, and three other enteric bacteria. The results showed that Yersinia pseudotuberculosis and enterocolitis showed a high titer of positive reaction (titer 1:27 ~ 1:10), the rest were negative, suggesting that: (1) Yop1 protein is Hyer (2) it is unlikely that the yop A gene mutated in Yersinia pestis will undergo a back-mutation, and further conclusions will be made after the extended detection. (3) Accordingly, it is reasonable to assume that the Yop1 protein single Clonal antibodies and their detection technology in the plague and Yersinia pseudotuberculosis and Yersinia enterocolitica O: 9 serotype and Brucella differential diagnosis has a good prospect.