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目的观察新生猪缺氧缺血性脑病模型肾动脉血流动力学改变及肾组织病理变化。方法 18只新生约克猪,雄性,猪龄3～5d,体质量2.0～2.5kg。随机分成对照组(n=8)和模型组(n=10)。模型组行气管插管、低氧通气及双侧颈动脉夹闭术,对照组不予低氧通气及双侧颈动脉夹闭。造模24h行肾脏超声检查,测量左、右肾动脉收缩期峰值血流速度(PSV)、舒张期末血流速度(EDV)及阻力指数(RI),取双侧平均值。对脑组织及肾组织行病理检测。结果与对照组相比,模型组PSV[(0.303±0.045)m/s vs(0.374±0.047)m/s]、EDV[(0.093±0.014)m/s vs(0.154±0.016)m/s]减低,RI(0.691±0.035 vs0.594±0.017)增高,差异有统计学意义(P<0.05)。模型组肾髓质部细胞肿胀、变性、坏死及凋亡;脑神经胶质细胞肿胀,神经元细胞变性及坏死。结论新生猪缺氧及脑缺血后早期除可直接引起脑损伤外,肾脏亦可出现低灌注,引起肾损伤。
Objective To observe the changes of renal artery hemodynamics and renal pathological changes in neonatal hypoxic-ischemic encephalopathy model. Methods 18 New York Yorkshire pigs, male, pig age 3 ~ 5d, body weight 2.0 ~ 2.5kg. Randomly divided into control group (n = 8) and model group (n = 10). Model group tracheal intubation, hypoxic ventilation and bilateral carotid artery occlusion, the control group without hypoxia and bilateral carotid artery occlusion. The kidneys were examined by sonography for 24 hours. The peak systolic velocity (PSV), end diastolic velocity (EDV) and resistance index (RI) of left and right renal arteries were measured. The bilateral averages were taken. Pathological examination of brain and kidney tissue. Results Compared with the control group, PSV [(0.303 ± 0.045) m / s vs 0.374 ± 0.047 m / s] and EDV [(0.093 ± 0.014) m / s vs (0.691 ± 0.035 vs0.594 ± 0.017), the difference was statistically significant (P <0.05). Nephomedial cells in the model group were swollen, degenerated, necrotic and apoptotic; brain glial cell swelling, degeneration and necrosis of neuronal cells. Conclusion Newborn pigs with hypoxia and early after cerebral ischemia can cause brain injury in addition to low perfusion of the kidneys, causing kidney damage.