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Objective To construct eukaryotic expression vector containing the HHV-8 K12 gene and green fluoresent protein gene. Methods The K12 gene of HHV-8 digested with EcoRI and XhoI was inserted into MCS sites of eukaryotic expression vector pCR3.1 to construct -pCR-K12; Green fluoresent protein(GFP) gene was amplified by polymerase chain reaction(PCR) from pGF-Puv as template, digested with KpnI and EcoRI and then inserted into upstream of K12 of pCR-K12 to construct pCR-GFP-K12. Results Restriction endonucleases digestion of pCR-GFP-K12 was the same as expected, in which K12 was fused with GFP and controlled by promoter CMV. Conclusion Construction of pCR-GFP-K12 was successful, which would benefit study on biology activity and action mechanism of HHV-8 K12.
Objective To construct eukaryotic expression vector containing the HHV-8 K12 gene and green fluoresent protein gene. Methods The K12 gene of HHV-8 digested with EcoRI and XhoI was inserted into MCS sites of eukaryotic expression vector pCR3.1 to construct-pCR-K12 ; Green Fluoresent protein (GFP) gene was amplified by polymerase chain reaction (PCR) from pGF-Puv as template, digested with KpnI and EcoRI and then inserted into upstream of K12 of pCR-K12 to construct pCR- GFP-K12. endonucleases digestion of pCR-GFP-K12 was the same as expected, where K12 was fused with GFP and controlled by promoter CMV. Conclusion Construction of pCR-GFP-K12 was successful, which would benefit study on biology activity and action mechanism of HHV -8 K12.