论文部分内容阅读
目的:探讨共表达HIV -1gp12 0与IFN- α重组鸡痘病毒诱导小鼠产生特异性的CTL杀伤活性。方法:将重组鸡痘病毒经肌肉注射免疫BALB c小鼠后,制备小鼠脾淋巴细胞悬液。以免疫小鼠的脾淋巴细胞为效应细胞,以表达HIV- 1结构蛋白的P815细胞为靶细胞,用乳酸脱氢酶释放法测定免疫小鼠脾特异性CTL杀伤活性。结果:重组病毒可有效地诱导特异性CTL的产生,且重组病毒免疫组小鼠脾特异性CTL对靶细胞的杀伤活性显著高于FPV对照组(P <0 .0 1)和PBS对照组(P <0. 0 1)。结论:共表达HIV- 1gp12 0和IFN -α的重组鸡痘病毒可激发强烈的细胞免疫,可作为我国HIV- 1疫苗候选株。
OBJECTIVE: To investigate the CTL killing activity induced by co-expressing HIV-1 gp12 0 and IFN-α recombinant fowlpox virus. Methods: BALB / c mice were immunized with recombinant fowlpox virus by intramuscular injection to prepare mouse spleen lymphocyte suspension. The splenic lymphocytes of immunized mice were used as effector cells. P815 cells expressing HIV-1 structural protein as target cells. The spleen-specific cytotoxic activity of CTLs was measured by lactate dehydrogenase release assay. Results: The recombinant virus could effectively induce the production of specific CTL. The cytotoxic activity of spleen-specific CTL on target cells in recombinant virus-immunized mice was significantly higher than that in FPV control group (P <0.01) and PBS control group P <0 0 1). Conclusion: The recombinant fowlpox virus co-expressing HIV-1 gp12 0 and IFN-α can stimulate strong cellular immunity and can be used as candidate strains of HIV-1 vaccine in our country.