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目的:检测人食管鳞癌组织中miR-181a-5P、miR-320a及TGF-β1的表达情况,研究其在食管鳞癌发生中的作用.方法:收集食管鳞癌及癌旁正常组织各3例,抽取总RNA,利用miRNA芯片技术检测miRNA的表达;采用qRT-PCR方法验证miRNA芯片结果的可靠性.采用软件和数据库对差异表达miRNA调控的靶基因进行初步分析.收集食管鳞癌及对照正常组织各22例,采用qRT-PCR方法检测miR-181a-5P、miR-320a和TGF-β1mRNA.结果: miRNA芯片结果显示,共有22种miR-NA在食管鳞癌和正常组织中的表达差异有统计学意义(P<0. 05),其中6种显著上调,16种显著下调;qRT-PCR证实miR-320a表达下降,miR-181a-5P表达上升,差异均有统计学意义( P<0. 05),与芯片结果一致; TGF-β1mRNA在食管鳞癌中表达较对照正常组织稍升高,但差异无统计学意义.结论: miR-181a-5P和miR-320a可能参与食管鳞癌的发生发展过程.“,”To investigate the exPression of miR-181a-5P, miR-320a and TGF-β1 in esoPhagea1 squamous ce11 carcino-ma and study their ro1es in the deve1oPment of esoPhagea1 squamous ce11 carcinoma. Methods: Three samP1es of esoPhagea1 squamous ce11 carcinoma and adjacent norma1 tissue were co11ected. The miRNA array was used to detect the exPression of miRNA. The re1iabi1i-ty of the miRNAs chiP resu1ts was verified using rea1-time quantitative Po1ymerase chain reaction (qRT-PCR). Differentia11y exPressed miRNA-regu1ated target genes were ana1yzed by software and database. miR-181a-5P, miR-320a and TGF-β1, extracted from 22 cases of esoPhagea1 squamous ce11 carcinoma and 22 cases of norma1 tissue, were verified using qRT-PCR. ResuIts: The miRNA chiP resu1ts showed that 22 miRNAs differentia11y exPressed in esoPhagea1 squamous ce11 carcinoma comPared with those in norma1 tissue ( P<0. 05). Six miRNAs significant1y increased, whi1e 16 significant1y decreased. It was confirmed that resu1ts of qRT-PCR were consistent with those of the miRNA array. qRT-PCR confirmed that the exPression of miR-320a decreased and that of miR-181a-5P increased in esoPhagea1 squamous ce11 carcinoma comPared with those in norma1 tissue ( P < 0. 05 ). The exPression of TGF-β1 in esoPhagea1 squamous ce11 carcinoma was s1ight1y higher than that in norma1 tis-sue, but the difference was not statistica11y significant. ConcIu- sion: miR-181a-5P and miR-320a may be engaged in carcinogenesis of esoPhagea1 squamous ce11 carcinoma.