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目的 探讨舒林酸硫化物及砜化物体外对血管内皮细胞增殖及凋亡的影响。方法 MTT法测定舒林酸硫化物及砜化物对人脐静脉内皮细胞增殖的作用 ,流式细胞仪和电镜分别检测其细胞周期、凋亡和超微结构的改变。结果 MTT显示 ,舒林酸硫化物可显著抑制血管内皮细胞的增殖 ,呈剂量依赖效应 ,IC50 为 2 0 0 μmol/L。流式细胞仪检测结果显示 ,舒林酸硫化物可改变血管内皮细胞的周期分布 ,G0 G1期 (对照组 77 74 %± 1 5 8% ,舒林酸砜化物组 75 6 3%± 2 12 % ,舒林酸硫化物组 4 6 12 %± 1 6 0 % ) ,S期 (对照组 13 6 4%± 1 2 2 % ,舒林酸砜化物组16 4 0 %± 2 30 % ,舒林酸硫化物组 2 7 2 6 %± 2 0 8% ) ,G2 M期 (对照组 8 6 1%± 0 6 7% ,舒林酸砜化物组 7 98%± 0 4 9% ,舒林酸硫化物组 2 6 6 2 %± 3 5 4% )。与对照组比较 ,舒林酸硫化物使G0 G1期细胞显著减少 (P <0 0 1) ,S期增加 (P <0 0 1) ,G2 M增加 (P <0 0 1)。舒林酸硫化物使细胞凋亡率增加 (对照组 6 0 8%± 3 39% ,舒林酸砜化物组 4 81%± 2 14 % ,舒林酸硫化物组 5 1 90 %± 5 6 7% ) ,与对照组比较 ,差异有极显著意义 (P <0 0 1)。电镜下舒林酸硫化物组可见细胞核染色质浓缩 ,细胞膜发泡 ,并出现凋亡小体。而舒林酸砜化
Objective To investigate the effects of sulindac sulfide and sulfone compounds on the proliferation and apoptosis of vascular endothelial cells in vitro. Methods MTT assay was used to determine the effects of sulindac sulfide and sulfonyl fluoride on the proliferation of human umbilical vein endothelial cells. The changes of cell cycle, apoptosis and ultrastructure were detected by flow cytometry and electron microscopy. Results MTT showed that sulindac sulfide could significantly inhibit the proliferation of vascular endothelial cells in a dose-dependent manner with IC50 of 200 μmol / L. Flow cytometry results showed that sulindac sulfide could change the periodic distribution of vascular endothelial cells, G0 G1 phase (77 74% ± 158% in control group, 75 6 3% ± 2 12 in sulindac sulfone group %, Sulindac sulfide group 4 6 12% ± 1 60%), S phase (13 6 4% ± 12 2% in the control group, 16 4 0% ± 2 30% in the sulindac sulfone group, (Sulfide Sulfide Group 2 7 2 6% ± 2 0 8%), G2 M phase (control group 8 6 1% ± 0 67%, sulindac sulfone group 7 98% ± 0 49% Sulfide group 2 6 6 2% ± 3 5 4%). Compared with the control group, sulindac sulfide reduced the number of G0 G1 phase cells significantly (P <0.01), S phase increased (P <0.01) and G2 M increased (P <0.01). Sulindac sulphide increased the rate of apoptosis (60.8% ± 399% in the control group, 81.1% ± 214% in the sulindac sulphone group, and 51 90% ± 5 6% in the sulindac sulphide group) 7%). Compared with the control group, the difference was significant (P <0.01). Under the electron microscope, sulindac sulfide group showed chromatin condensation, cell membrane foaming, and apoptotic bodies. The sulindac sulfone