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目的:观察不同栽培品种、不同加工方法的温莪术挥发油的化学成分及其对肿瘤细胞的生长抑制作用的差异。方法:利用MTT法检测同一产地不同栽培品种、不同加工方法的温莪术的挥发油对胃癌肿瘤细胞MGC-803增殖的影响,并用GC-MS检测出挥发油中各成分的相对含量。结果:不同栽培品种、不同加工方法的温莪术挥发油对MGC-803的生长抑制作用存在着明显的差异,当温莪术提取的挥发油为高浓度时,组培品种鲜品对MGC-803的生长抑制最高,为20.2%;当药液浓度为低浓度时,普通品种鲜品对MGC-803的生长抑制最高为18.4%。其所含化学成分也差异明显,如β-水芹烯、双环[3.1.1]庚烷,6,6-二甲基-2-亚甲基(1S)是传统鲜品的特征性成分,环己烷,1-乙烯基-1-甲基-2-(1-甲基乙烯基)-4-(1-甲基亚乙基)为普通鲜品的特征性成分;双环[3.1.0]六角-2-烯,4-甲基-1-(1-甲基乙基)-,β-蒎烯中,为组培鲜品其特征性成分。结论:不同栽培品种、不同加工方法对其挥发油类成分在含量和组成上的差异较大,对于抑制胃癌肿瘤细胞的增殖作用上也存在明显差异,以组培品种的鲜品与加工品的挥发油对MGC-803的抑制作用最强。
OBJECTIVE: To observe the chemical composition of volatile oil from Curcuma curcumae cultivated with different cultivars and different processing methods and their effects on the growth inhibition of tumor cells. Methods: MTT assay was used to detect the effects of different volatile oils from different cultivars and processing methods on the proliferation of gastric cancer cell line MGC-803. GC-MS was used to detect the relative content of each component in the volatile oil. Results: The growth inhibition of MGC-803 by different cultivars and different processing methods was significantly different. When the volatile oil extracted by Curcuma longa was high concentration, the growth inhibition of MGC-803 The highest was 20.2%. When the liquid concentration was low, the growth of MGC-803 was up to 18.4%. Its chemical composition is also significantly different, such as β-phellandrene, bicyclo [3.1.1] heptane, 6,6-dimethyl-2-methylidene (1S) is a characteristic feature of traditional fresh products, Cyclohexane and 1-vinyl-1-methyl-2- (1-methylvinyl) -4- (1-methylethylidene) ] Hexa-2-ene, 4-methyl-1- (1-methylethyl) -, β-pinene, the characteristics of tissue culture fresh ingredients. CONCLUSION: Different cultivars and processing methods have significant differences in the content and composition of the volatile oil components. There are also significant differences in the inhibition of the proliferation of gastric cancer cells. Using the fresh and processed oil of the cultivated cultivars, The inhibitory effect of MGC-803 strongest.