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采用人工繁殖带毒褐飞虱( Nelaparvata Lugens) 攻毒接种,结合症状观察和RTPCR 检测方法,对57 个含有水稻齿叶矮缩病毒(RRSV) S7 ,S9 和S10 基因片段的转基因水稻对水稻齿叶矮缩病毒的抗性进行了评价。总体而言,大多数转基因稻发病率比受体品种的发病率要低。从中初步得到对RRSV 具有较强抗性的水稻转基因系315 和316 ,其发病率分别为15 .8 % 和13 .3 % ;具有中等抗性的水稻转基因系184 和328 ,发病率分别为26 .9 % 和28 .0 % ;而对照受体品种和当地感病品种的发病率则分别为100 % 和84 .2 % 。将RTPCR 的方法用于转基因稻抗性的检测,可提高检测的灵敏度。
Fifty-seven transgenic rice plants containing the S7, S9 and S10 gene fragments of RRSV were inoculated with Nelaparvata Lugens inoculated with artificial propagation and observed by RT-PCR and RT-PCR. Leaf dwarf virus resistance was evaluated. Overall, the incidence of most GMO rice is lower than that of recipient varieties. The rice lines 315 and 316 with strong resistance to RRSV were obtained from them, and their incidence rates were 15 and 15 respectively. 8% and 13. 3%, respectively. The moderately resistant rice lines 184 and 328 had a morbidity of 26%. 9% and 28. 0%, while the incidences of control and local susceptible varieties were 100% and 84% respectively. 2%. RT-PCR method for the detection of transgenic rice resistance, can improve the detection sensitivity.