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目的:建立复方紫青膏的质量标准。方法:用薄层色谱法(TLC)对紫草、大黄、冬青进行定性鉴别,用高效液色谱法对膏剂中左旋紫草素进行含量测定。结果:TLC斑点圆整,分离度好,易于区别;左旋紫草素进样量在0.2032-1.2192μg的范围内与峰面积线性关系良好(r=0.9999),平均回收率为99.5%,RSD=1.7%(n=6)。结论:所用方法简便准确、重复性好,所建标准可用于复方紫青膏的质量控制。
Objective: To establish the quality standard of compound purple plaster. Methods: Lithospermum, rhubarb and holly were qualitatively identified by TLC. The content of L-shikonin in the ointment was determined by HPLC. Results: TLC spots were well rounded and the separation was easy and easy to distinguish. The injection volume of levosulfan was linear with the peak area in the range of 0.2032-1.2192μg (r = 0.9999), the average recovery was 99.5%, RSD = 1.7% (n = 6). Conclusion: The method is simple and accurate, and the repeatability is good. The standard can be used for the quality control of compound purple plaster.