论文部分内容阅读
将A20淋巴瘤细胞,反复冻融,获得肿瘤细胞裂解物的抗原肽,以来源于结核分枝杆菌(Mycobacterium tuberculosis)热休克蛋白70(HSP70)第407-426(mHSP70407~426,M)的两段串联重复序列M2小肽为佐剂,通过双功能偶联试剂戊二醛偶联制备肿瘤细胞疫苗A20-M2,偶联产物在体外修饰未成熟的DC,通过检测负载肿瘤细胞抗原的DC细胞分泌的细胞因子以及抗原来确定被修饰DC细胞的成熟度,获得成熟DC即获得DC疫苗。用A20-M2与DC两种疫苗联合给药治疗小鼠,实验结果显示,激发的免疫应答对于A20肿瘤攻击起到有效的保护作用,与PBS阴性对照组比较,平均肿瘤重显著降低(P<0.01),通过ELISA法,从小鼠血清中检测到高滴度抗细胞裂解物类抗体,淋巴细胞增殖试验显示疫苗联合给药后能够有效的刺激脾淋巴细胞的增殖反应。A20-M2能够有效的刺激DC细胞成熟,A20-M2与成熟DC细胞联合给药能够有效的抑制小鼠A20淋巴瘤的生长。
A20 lymphoma cells were repeatedly frozen and thawed to obtain tumor cell lysate antigen peptides derived from Mycobacterium tuberculosis heat shock protein 70 (HSP70) 407-426 (mHSP70407 ~ 426, M) The tandem repeat M2 peptide was adjuvant, and the tumor cell vaccine A20-M2 was prepared by conjugating glutaraldehyde, a bifunctional coupling reagent. The conjugate was used to modify immature DCs in vitro. By detecting DC cells carrying tumor cell antigen Secreted cytokines and antigens to determine the maturity of modified DC cells to obtain mature DC to obtain DC vaccine. The combination of A20-M2 and DC vaccine in mice, the experimental results show that the immune response to challenge A20 tumor attack has played an effective protective effect, and PBS negative control group, the average tumor weight was significantly lower (P < 0.01). High titer anti-cell lysate antibody was detected in mouse serum by ELISA. The lymphocyte proliferation test showed that the combination of the vaccine and the vaccine could effectively stimulate the proliferation of splenic lymphocytes. A20-M2 can effectively stimulate DC cell maturation, A20-M2 combined with mature DC cells can effectively inhibit the growth of mouse A20 lymphoma.