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目的通过检测甘南地区部分体检者血清中TTV,分析TTV在该地区的流行情况及特征。方法随机收集甘南州人民医院藏族体检者血清362份,巢式聚合酶链式反应检测血清中TTV-DNA;抽取8份阳性血清扩增产物测序并进行同源性比较和系统进化分析。结果 362份血清中,阳性血清128份(男性81份、女性47份),阳性率为35.4%(128/362),男性与女性之间TTV感染差异具有统计学意义(P<0.05)。序列同源性分析表明,8份阳性血清序列与日本参考株、北京株和深圳株对应核苷酸序列的同源性在88.7%-100%;分子进化树分析显示,8个序列分布在2个不同的分支中,7个序列与北京株、日本参考株TA278同属1个分支,1个序列与深圳株组成另1个分支,且其同源性为99.9%,提示其为同一型或亚型。结论这一结果表明在甘南藏族人群中存在TTV感染,将为预防甘南地区TTV流行及其与其他疾病的相关性研究奠定基础。
Objective To detect the prevalence and characteristics of TTV in the area by detecting TTV in sera of some medical people in Gannan area. Methods 362 serum samples of Tibetans from Gannan People’s Hospital were collected randomly. TTV-DNA in serum was detected by nested polymerase chain reaction (PCR). Eight positive serum samples were sequenced and their homologies and phylogenetic analyzes were performed. Results Among the 362 serum samples, 128 were positive (81 males and 47 females), the positive rate was 35.4% (128/362). There was significant difference in TTV infection between males and females (P <0.05). Sequence homology analysis showed that the homology of 8 positive serovars with that of reference strains, Beijing strains and Shenzhen strains was 88.7% -100%. The molecular phylogenetic tree analysis showed that the 8 sequences were distributed in 2 Among the seven branches, seven sequences belong to one branch of Beijing and Japan reference strains TA278, one sequence and Shenzhen strain form another branch, and the homology is 99.9%, suggesting that it is the same type or subtype type. Conclusions This result indicates that the presence of TTV infection among Tibetan people in Gannan will lay a foundation for the study on the correlation between TTV prevalence in Gannan and other diseases.