论文部分内容阅读
研究了(CHR)在栉孔扇贝(Chlamys farreri)性腺成熟过程中累积含量的变化和损伤效应。结果表明:CHR对雌雄栉孔扇贝组织中CHR累积含量、MDA(丙二醛)含量、PC(蛋白质羰基)含量和DNA链断裂(F值)影响显著(P<0.05),而对照组无明显差异(P>0.05)。各处理组雌、雄栉孔扇贝卵巢、精巢和软体部CHR累积含量的变化趋势基本相同,均在10d内呈逐渐升高趋势,并呈现明显的剂量效应,而且雌、雄栉孔扇贝CHR含量表现为卵巢>精巢?软体部。各处理组雌性栉孔扇贝卵巢MDA含量在6d内呈峰值变化,于3d时达到最大值,6d后保持稳定,且显著高于对照组水平,而卵巢PC含量、F值在10d内分别呈升高或下降趋势,其中卵巢PC含量表现出明显的剂量效应;低、中浓度处理组雄性栉孔扇贝精巢MDA含量在10d内逐渐升高,而高浓度组在6d内呈峰值变化,在3d时达到最大值,6d后趋于稳定,且明显高于对照组水平,精巢PC含量、F值在10d内与卵巢的变化趋势类似,但未呈现明显的剂量效应。由此可见,栉孔扇贝在CHR胁迫下性腺累积含量较高,导致性腺脂质、蛋白质和DNA受到严重损伤,产生明显的生殖毒性效应,其中卵巢PC含量可作为栉孔扇贝性腺损伤的潜在指标之一。
The change of cumulative content and damage effect of (CHR) on gonadal maturation in Chlamys farreri were studied. The results showed that CHR had a significant effect on CHR accumulation, MDA content, PC (protein carbonyl) content and DNA strand breaks (F <0.05), but not in the control group Difference (P> 0.05). The CHR content in ovary, testis and soft body of female and male Zhirong scallop in different treatment groups showed the same trend, and all tended to increase gradually within 10 days and showed obvious dose-effect, and CHR content of female and male Zhikong scallop The performance of the ovary> testis? Software Department. The content of MDA in the ovaries of female Chlamys farreri in each treatment group peaked within 6 days, reaching the maximum at 3 days and remained stable 6 days later, which was significantly higher than that of the control group The content of MDA in ovary PC showed a dose-dependent manner. The content of MDA in testis of male and female chlamys farreri increased gradually within 10d, while the concentration in high concentration group peaked within 6d. At 3d Reaching the maximum after 6 days, and stabilized at 6 days, which was significantly higher than that of the control group. PC content and F value of the testis were similar to those of the ovary within 10 days, but showed no significant dose effect. Thus, Chlamys farreri CHR stress in the gonads accumulated higher levels, resulting in gonadal lipid, protein and DNA were severely damaged, resulting in significant reproductive toxicity effects, including ovarian PC content can be used as a potential indicator of gonadal injury in Chlamys farreri one.