论文部分内容阅读
该文采用亲和指数法(种子数/自交授粉花朵数)分别在羽衣甘蓝‘赤兔’和‘白波’栽培品种的自交后代中,经过3年的自交选育获得1个自交不亲和系4·1·1和一个自交亲和系3·2·3·由于在芸苔属植物F1代杂交种的生产和自交不亲和分子机制的研究方面S单倍型鉴定是必要的,因此在该文中我们采用了PCR方法利用引物PK1和PK4获得了BoSRKx的基因组序列,此序列的大小为919bp.经过数据库检索比对后发现BoSRKx的序列与BoSRK13b的序列完全一致.通过反转录PCR的方法获得了BoSRKx的cDNA序列,结果发现BoSRKx和BoSRK13b的序列在转录水平上也是完全一致的;另一方面,我们采用SCR保守信号肽编码区和NotⅠ-oligo(dT)设计的引物获得了BoSCRx的cDNA序列,经过数据库的检索比对后发现BoSCRx的序列比BoSCR13的序列只多出3个连续的碱基.根据上述结果我们推断出Sx单倍型就是S13b单倍型;最后,通过遗传分析结合PCR-RFLP方法和DNAblot方法对自交不亲和系进行了S13b纯合体的鉴定.
In this study, self-fertilizing progeny of Kaili ’Red Rabbit’ and ’White Cultivars’ cultivars were used to obtain one selfing Incompatibility 4.1.1 and an inbred lineage 2.3.2 Identification of S haplotypes in the generation of F1 hybrids in Brassica and in the study of self-incompatibility molecular mechanisms Therefore, in this paper, we used PCR method to obtain the genomic sequence of BoSRKx using primers PK1 and PK4, and the size of this sequence is 919bp. After searching the database, we found that the sequence of BoSRKx is exactly the same as the sequence of BoSRK13b. RT-PCR method was used to obtain the cDNA sequence of BoSRKx. The results showed that the sequences of BoSRKx and BoSRK13b were exactly the same at the transcriptional level. On the other hand, we designed the sequence of the conserved signal peptide of Notch and NotⅠ-oligo (dT) Primers obtained the cDNA sequence of BoSCRx, after the database search comparison found that BoSCRx sequence than BoSCR13 sequence only three consecutive bases.According to the above results we conclude that the Sx haplotype is the S13b haplotype; Finally , Through genetic points PCR-RFLP method and the binding method DNAblot self-incompatible lines were identified S13b homozygous.