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通过田间和实验室相结合把低木质素、高消化率基因bmr和光周期敏感基因PS聚合到饲草高粱中,以提高饲草高粱的产量和品质。以含PS基因的EBA-3和含bmr基因的Tx623B配制杂交组合,构建了F2代分离群体。根据表型分离比例分析,光敏和褐色中脉可能分别受两对彼此独立的非等位基因控制,并且基因之间存在着互作。光敏基因之间存在互补作用,褐色中脉基因之间存在抑制作用。根据高粱的遗传连锁图谱,选取相应的SSR标记。经连锁遗传分析,找到了2个SSR标记,Xtxp7与bmr基因连锁,Xtxp20与PS基因连锁。由于Xtxp7位于连锁群B,Xtxp20位于连锁群G,据此认为高粱有一个bmr基因位于B染色体上和有一个PS基因位于G染色体上。这两个标记的获得可用于标记辅助育种,加速杂交种的转育与利用。此外,对影响开花时间的QTLs和主效基因在高粱和水稻上可能一致进行了讨论。
Through the field and laboratory combination of low lignin, high digestibility genes bmr and photoperiod sensitive gene PS polymerization forage sorghum, in order to improve the yield and quality of forage sorghum. The F2 generation segregation population was constructed with EBA-3 containing PS gene and Tx623B with bmr gene. According to phenotypic segregation analysis, the light-sensitive and brown midrib may be controlled by two independent pairs of non-alleles, respectively, and the genes interact with each other. Photosensitive genes complement each other, there is inhibition between brown midrib genes. According to the genetic linkage map of sorghum, select the corresponding SSR markers. After linkage analysis, two SSR markers were found, Xtxp7 was linked with bmr gene and Xtxp20 was linked with PS gene. Since Xtxp7 is located in linkage group B and Xtxp20 is located in linkage group G, it is considered that there is a bmr gene in sorghum located on chromosome B and a PS gene located on chromosome G. The acquisition of these two markers can be used to mark assisted breeding and accelerate the breeding and utilization of hybrids. In addition, QTLs and major genes affecting flowering time may be discussed consistently on sorghum and rice.