论文部分内容阅读
[目的]针对细菌培养体的小鼠喂养试验结果,建立细菌培养体中霉菌毒素的系统筛选方法。[方法]采用霉菌毒素净化萃取柱选择性地进行样品净化处理,经高速液相色谱技术与高分辨质谱技术(RRLC-TOF)测定,用相关代谢组学软件系统处理分析。[结果]采用液相色谱与高分辨质谱技术方法,对细菌培养前后能识别的标志性组分最少达15000个;而采用代谢组学方法将培养前后的差异组分数量缩小到<2000个,再结合霉菌毒素软件库的查询,进而确认培养后的细菌培养体中新出现了镰刀菌烯醇(fusarenon X)、蛇形菌素(diacetoxyscirpenol)、15-乙酰蛇形菌素(15-acetoxyscirpenol)等毒素。[结论]应用代谢组学的相关技术方法,实现对宏量数据中差异性组分快速筛选,并为分析毒理学提供了一个新的技术方法。
[Objective] The aim of this study was to establish a systematic screening method for mycotoxins in bacterial culture against the experimental results of mice feeding in bacterial culture. [Method] The mycelium was purified by mycotoxin purification column. The samples were cleaned up by high performance liquid chromatography (HPLC) with high resolution mass spectrometry (RRLC-TOF) and analyzed by the relevant metabolomics software system. [Result] The results showed that there were at least 15000 identifiable components before and after bacterial culture by using liquid chromatography and high resolution mass spectrometry, while the number of differential components before and after culturing was reduced to <2000 by using metabolomics method. Combined with the query of mycotoxin software library, it was confirmed that fusarenon X, diacetoxyscirpenol, 15-acetoxyscirpenol, Other toxins. [Conclusion] The application of metabonomics related techniques and methods to achieve rapid screening of differential components in the macro-data, and to provide a new technical method for the analysis of toxicology.