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目的探讨肝X受体(LXR)及其表达基因胆固醇酯转运蛋白(CETP)、环氧化酶2(COX2)在学龄期肥胖儿童中的表达及其意义。方法 2011年6月至10月以计算机随机系统随机抽取兰州市4个区8所小学1~6年级7~14岁学龄期儿童为研究对象,通过体格发育指标检测,筛选出肥胖儿童80例作为研究组,并进行肥胖和超重分组;同时选取年龄匹配、体重指数(BMI)正常的51名儿童作为对照组。取晨血分离培养巨噬细胞,采用实时荧光定量聚合酶链反应(FQ-PCR)技术检测LXR、CETP和COX2相对表达量。结果肥胖组与超重组LXR、CETP和COX2表达量高于对照组,差异有统计学意义(P均<0.05)。肥胖组与超重组LXR、CETP和COX2表达量差异无统计学意义(P均>0.05)。研究组中不同性别LXR、CETP和COX2表达量差异无统计学意义(P均>0.05)。评价指标LXR分别与COX2和CETP呈正相关(P均<0.05)。结论肥胖可导致学龄期儿童体内LXR、CETP和COX2表达量上调;LXR、CETP和COX2表达量高低与肥胖程度有关;学龄期儿童LXR、CETP和COX2表达不受性别影响。
Objective To investigate the expression and significance of liver X receptor (LXR) and its express genes cholesterol ester transporter (CETP) and cyclooxygenase 2 (COX2) in school-aged obese children. Methods From June to October of 2011, randomly selected random numbers from 8 primary schools of Grade 1 to Grade 6 school aged 7-14 in 4 districts of Lanzhou City for study. Eighty obese children were screened out through physical development test. The study group was divided into obesity and overweight group. At the same time, 51 children with normal age and body mass index (BMI) were selected as the control group. The macrophages were isolated and cultured in morning blood. The relative expression levels of LXR, CETP and COX2 were detected by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR). Results The expression of LXR, CETP and COX2 in obesity group and overweight group was higher than that in control group (P <0.05). The expression of LXR, CETP and COX2 in obesity group and overweight group had no significant difference (all P> 0.05). There was no significant difference in the expression of LXR, CETP and COX2 among the study groups (P> 0.05). The evaluation index LXR was positively correlated with COX2 and CETP respectively (all P <0.05). Conclusions Obese can lead to the up-regulation of LXR, CETP and COX2 expression in school-age children. The expressions of LXR, CETP and COX2 are related to the degree of obesity. The expression of LXR, CETP and COX2 in school-age children are not affected by gender.