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目的:分析急性非淋巴细胞白血病(acute nonlymphocytic leukemia,ANLL)的核型状况。方法:利用短期培养法和直接法制备骨髓细胞染色体,用R带显带技术对151例初治的ANLL患者行染色体核型检查。结果:该组患者中克隆性染色体异常的有84例(56%)。异常核型主要为特异性染色体重排,共有65例,占核型异常的77%。t(15;17)(35例)和t(8;21)(23例)是最常见的结构异常。三体-8是最常见的数目异常。100%的t(7;11)见于M2,100%的t(15;17)见于M3,83%的t(8;21)见于M2,100%的t/del(11q23)见于M5。结论:应用R带显带技术,56%的ANLL患者可检出克隆性染色体异常特别是特异性染色体重排。它们和FAB分型相关,因此核型是ANLL诊断、分型、判断预后的一项重要指标。
Objective: To analyze the karyotype of acute nonlymphocytic leukemia (ANLL). Methods: Chromosomes of bone marrow cells were prepared by short-term culture method and direct method. Chromosomal karyotype examination was performed on 151 newly diagnosed ANLL patients by R-banding technique. Results: There were 84 cases (56%) of clonal chromosomal abnormalities in this group of patients. Abnormal karyotypes are mainly specific chromosomal rearrangements, a total of 65 cases, accounting for 77% of karyotype abnormalities. t (15; 17) (35 cases) and t (8; 21) (23 cases) were the most common structural abnormalities. Trisomy-8 is the most common number anomaly. 100% t (7; 11) found in M2, 100% t (15; 17) found in M3, 83% t (8; 21) found in M2, 100% t / del (11q23) found in M5. CONCLUSIONS: Clonal chromosomal abnormalities, particularly specific chromosomal rearrangements, can be detected in 56% of ANLL patients using R-banding. They are related to FAB typing, so karyotype is an important indicator of ANLL diagnosis, classification and prognosis.