论文部分内容阅读
[目的]通过利用SSR分子标记技术测定向日葵种子纯度,以期为生产加工应用提供准确、便捷的杂交种种子纯度鉴定方法提供依据。[方法]该研究以新食葵6号及其双亲的DNA为模板,通过DNA提取、PCR扩增反应和制作电泳的步骤,进行了约100对SSR物分子标记的筛选。[结果]SSR多态引物标记532在新食葵6号母本分别产生特异条带大小为496bp,父本特异标记条带大小为451 bp;引物标记574在母本上特异条带大小为364 bp,父本上的特异标记条带大小为384 bp,同时室内分子鉴定的纯度和田间鉴定纯度基本一致;通过SSR分子标记方法可出可得到区分父本、母本和杂交种的引物标记532和574,这2个引物标记可有效用于鉴定上述新食葵6号杂交种种子的纯度,辨别种子的真伪。[结论]该方法具有简单,快速、准确、重演性高的优点,目前该方法已成为向日葵品种鉴定的主要方法。
[Objective] The aim of the study was to determine the purity of sunflower seeds by using SSR molecular markers and to provide a basis for the accurate and convenient hybrid seed purity identification methods for production and processing applications. [Method] About 100 pairs of SSR molecular markers were screened by DNA extraction, PCR amplification and electrophoresis. [Result] The specific band size of 496bp and 451 bp in the male parent of Xifan Kwai 6 were detected by SSR polymorphism primer 532. The specific band size of primer 574 in the female parent was 364 bp, the size of the specific marker band on the male was 384 bp, while the purity of the indoor molecular identification was basically the same as that of the field identification; primer markers 532 for distinguishing between male and female parents and hybrids were obtained by the SSR molecular marker method And 574, respectively. These two primer markers can be effectively used to identify the purity of the seed of the new Nongfu No. 6 hybrid and to distinguish the authenticity of the seeds. [Conclusion] The method has the advantages of simple, rapid, accurate and reproducible. At present, this method has become the main method of identifying sunflower varieties.