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目的检测肺癌组织、相应的癌旁组织及正常肺组织中E-cadherin基因启动子CpG岛甲基化水平,探讨肺癌的发病机制。方法采用甲基化特异性PCR技术检测22例肺癌组织、相应的癌旁组织和9例正常肺组织中E-cad-herin基因启动子CpG岛甲基化水平。结果肺癌中E-cadherin基因启动子CpG岛完全甲基化率为13.6%(3/22),部分甲基化率为27.3%(6/22),总甲基化率为40.9%(9/22),显著高于相应癌旁组织中该基因的甲基化率9.1%(2/22)。9例正常肺组织中该基因未发生甲基化。此外,E-cadherin基因启动子CpG岛异常甲基化与患者性别、年龄无关,甲基化的发生率随着肿瘤的分期早晚有上升的趋势。结论E-cadherin基因启动子CpG岛的异常甲基化是肺癌发生中的常见分子事件,可能参与了肺癌的发生发展过程。
Objective To detect the methylation level of CpG island in promoter region of E-cadherin gene in lung cancer tissues, corresponding paracancerous tissues and normal lung tissues and to explore the pathogenesis of lung cancer. Methods Methylation-specific PCR was used to detect the methylation level of CpG island in E-cad-herin gene promoter in 22 cases of lung cancer, corresponding paracancerous tissues and 9 cases of normal lung tissues. Results The complete methylation rate of CpG island of E-cadherin gene promoter in lung cancer was 13.6% (3/22), the partial methylation rate was 27.3% (6/22) and the total methylation rate was 40.9% (9 / 22), which was significantly higher than that of corresponding paracancerous tissues (9.1% (2/22)). The methylation was not found in 9 normal lung tissues. In addition, abnormal methylation of CpG island of E-cadherin gene promoter has nothing to do with gender and age of patients, and the incidence of methylation tends to rise sooner or later with the staging of tumor. Conclusion The abnormal methylation of CpG island of E-cadherin promoter is a common molecular event in lung carcinogenesis and may be involved in the development and progression of lung cancer.