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目的 观察NGF GDNF基因修饰神经干细胞 (NSC)单独和联合移植对穹窿海马伞 (FF)切断后胆碱能神经元的保护作用。 方法 将两种基因修饰的NSC单独和联合移植入FF切断的大鼠侧脑室。移植后 3周取脑行ChAT免疫组织化学染色 ,用Student NewmanKaels方法对内侧隔核 (MS)和斜角带垂直支 (VDB)的ChAT阳性细胞数 (损伤侧与正常侧的百分比 )进行计数分析。 结果 NGF组伤侧MS的ChAT阳性神经元数为 81% ,明显高于损伤组 (34% )、NSC组 (36 % )和GDNF组 (5 0 % ) ,P <0 0 1;NGF +GDNF组为 6 8% ,明显高于损伤组和NSC组 (P <0 0 1) ,亦高于GDNF组 (P <0 0 5 ) ;GDNF组与损伤组和NSC组相比 ,有统计学意义 (P <0 0 5 )。NGF组和NGF +GD NF组伤侧VDB的ChAT阳性神经元数分别为 80 %和 72 % ,明显高于损伤组 (5 6 % )和NSC组 (5 2 % ) ,P <0 0 1,亦高于GDNF组 (5 9% ) ,P <0 0 5 ;GDNF组与损害组和NSC组相比无明显差异 (P >0 0 5 )。 结论 NGF GDNF基因修饰NSC单独和联合移植均能不同程度地保护损伤的ChAT阳性神经元 ,NGF组和NGF +GDNF组作用较大 ,GDNF组作用较小。
Objective To observe the protective effects of NGF GDNF gene-modified neural stem cells (NSCs) alone and in combination on the cholinergic neurons after Fimbrial Fimbraction (FF). Methods Two genetically modified NSCs were individually and co-transplanted into FF-severed lateral ventricle of rats. ChAT immunohistochemistry was performed 3 weeks after transplantation, and the number of ChAT positive cells (percentage of lesion side vs. normal side) in medial septum (MS) and vertical diagonal branch (VDB) was counted by Student NewmanKaels method . Results The number of ChAT positive neurons in injured MS group was 81%, which was significantly higher than that in injury group (34%), NSC group (36%) and GDNF group (50%), P <0.01; NGF + GDNF Group (68%) was significantly higher than the injury group and the NSC group (P <0.01), also higher than the GDNF group (P <0 05); GDNF group compared with the injury group and NSC group was statistically significant (P <0 05). The numbers of ChAT positive neurons in VDB of NGF group and NGF + GD NF group were 80% and 72%, respectively, which were significantly higher than that of NSC group (52% vs 52%, P <0.01) Also higher than GDNF group (59%), P <0 05; there was no significant difference between GDNF group and injury group and NSC group (P> 0.05). Conclusion NGF GDNF gene-modified NSC can protect damaged ChAT positive neurons to some extent, both in NGF group and NGF + GDNF group, and in GDNF group.