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本文旨在探讨胆碱能神经在脑缺血再灌注时的变化和意义。采用双侧颈总动脉夹闭（CCAO）的大鼠脑缺血动物模型，用改良的Ellman法测定大脑皮层、海马、间脑、纹状体和脑子五个脑区乙酰胆碱酯酶（AChE）活性，观察脑缺血30分钟和再灌注30分钟不同脑区AChE活性的变化。结果发现：在生理状态下不同胞区AChE活性不同，纹状体最高（181．8±1．7μ／g），皮层最低（24．1±0．4μ／g），CCAO30分钟皮层、海马、间脑、纹状体的AChE活性均明显下降，较正常大鼠分别降低278％、26．3％、16．9％、23．6％（P＜0．01），再灌注30分钟后分别回升18.4％、17.6％、10．2％、23．7％（P＜0．01）。而脑干在缺血和再灌注中无显著变化。结果提示不同胞区胆碱能活性程度不同；脑AChE活性对缺血和再灌注是敏感的，推测胆碱能神经可能在缺血性脑损伤中起重要作用。
This article aims to explore the change of cholinergic nerve in cerebral ischemia-reperfusion and its significance. The cerebral ischemia model of rats with bilateral common carotid artery occlusion (CCAO) was established. The acetylcholinesterase (AChE) activity in cerebral cortex, hippocampus, diencephalon, striatum and brain was measured by a modified Ellman method The changes of AChE activity in different brain regions were observed at 30 minutes after cerebral ischemia and 30 minutes after reperfusion. The results showed that the activity of AChE in different cell regions was different in the physiological state, with the highest striatum (181.8 ± 1.7μ / g), the lowest cortex (24.1 ± 0.4μ / g), CCAO 30minutes, hippocampus, The activity of AChE in diencephalon and striatum were significantly decreased by 278%, 26.3%, 16.9% and 23.6% respectively compared with those in normal rats (P <0.01). After reperfusion for 30 minutes Rose 18.4%, 17.6%, 10.2%, 23.7% (P <0.01). There was no significant change in the brainstem between ischemia and reperfusion. The results suggest that the degree of cholinergic activity varies in different regions. The activity of brain AChE is sensitive to ischemia and reperfusion. It is speculated that cholinergic nerve may play an important role in ischemic brain injury.