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目的:通过研究大鼠急性细菌性前列腺炎组织中腺苷浓度以及TNF-α、IL-10的表达,探讨腺苷与炎症细胞因子在前列腺急性炎症过程中的作用。方法:建立急性细菌性前列腺炎大鼠模型,分为前列腺炎组、茶碱干预组、假手术组和空白对照组,每组12只。分别于4、14d处死半数并获取前列腺组织。免疫组化检测组织中IL-10、TNF-α的表达,高效液相色谱法检测各组组织中的腺苷浓度。结果:急性细菌性炎症环境下前列腺组织中不同处理时间的腺苷浓度[(48.38±17.27)、(26.54±11.22)μg/g]均显著高于假手术组[(0.45±0.25)、(0.46±0.29)μg/g]和空白对照组[(0.41±0.23)、(0.43±0.27)μg/g,P<0.05];TNF-α(0.23±0.08、0.21±0.03)、IL-10(0.13±0.03、0.25±0.01)的表达较假手术组(0.07±0.03、0.07±0.01;0.07±0.03、0.07±0.03)、空白对照组(0.07±0.06、0.07±0.06;0.07±0.01、0.07±0.02)显著升高(P<0.05);给予腺苷受体阻滞剂茶碱干预后,与前列腺炎组相比,干预组大鼠的前列腺组织炎症程度加重,组织中腺苷浓度显著升高[(86.64±32.87)、(51.17±22.96)μg/g,P<0.05],TNF-α的表达显著升高(0.37±0.08、0.32±0.06,P<0.05),但IL-10的表达无明显改变(0.12±0.06、0.15±0.06,P>0.05)。结论:腺苷可能通过腺苷受体信号通路调节大鼠急性细菌性前列腺炎组织中细胞因子IL-10、TNF-α的表达,影响组织炎症的进程。
OBJECTIVE: To investigate the role of adenosine and inflammatory cytokines in acute prostatic inflammation in rats by studying the concentration of adenosine and the expression of TNF-α and IL-10 in acute bacterial prostatitis tissues of rats. Methods: Acute bacterial prostatitis rat model was established and divided into prostatitis group, theophylline intervention group, sham operation group and blank control group, 12 rats in each group. Half of them were sacrificed on 4,14d and prostate tissue was obtained. Immunohistochemistry was used to detect the expression of IL-10 and TNF-α in tissues. The concentration of adenosine in each tissue was detected by high performance liquid chromatography. Results: The concentrations of adenosine at different treatment time in prostate tissue [(48.38 ± 17.27), (26.54 ± 11.22) μg / g] in acute bacterial inflammation were significantly higher than those in sham operation group [(0.45 ± 0.25), (0.23 ± 0.08,0.21 ± 0.03), IL-10 (0.13 ± 0.27) μg / g, P <0.05) ± 0.03,0.25 ± 0.01) were significantly higher than those in the sham operation group (0.07 ± 0.03,0.07 ± 0.01; 0.07 ± 0.03,0.07 ± 0.03), the blank control group (0.07 ± 0.06,0.07 ± 0.06; 0.07 ± 0.01,0.07 ± 0.02) (P <0.05). After intervention of theophylline adenosine receptor blocker, the degree of inflammation of the prostate tissue in the intervention group was increased compared with the prostatitis group, and the concentration of adenosine in the tissue was significantly increased [ (86.64 ± 32.87), (51.17 ± 22.96) μg / g, P <0.05]. The expression of TNF-α was significantly higher than that of the control group (0.37 ± 0.08,0.32 ± 0.06, P <0.05) Change (0.12 ± 0.06,0.15 ± 0.06, P> 0.05). CONCLUSION: Adenosine can regulate the expression of cytokines IL-10 and TNF-α in acute bacterial prostatitis through adenosine receptor signaling pathway and affect the process of tissue inflammation.