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目的:用衰减全反射傅立叶红外光谱考察壳聚糖(CS)及其衍生物对小鼠角质层含水量的影响,以初步探讨其作为透皮吸收促进剂的作用机理。方法:合成不同取代度的N-三甲基壳聚糖(TMC)和N-羧甲基壳聚糖(MCC);酶消化法提取小鼠角质层,分别用2.5%TMC、MCC、CS溶液处理之后进行ATR-FTIR分析,计算酰胺基Ⅰ吸光度和酰胺基Ⅱ吸光度之比。结果:TMC20,TMC40,TMC60,MCC,CS,H_2O(作为对照)处理之后,角质层酰胺基Ⅰ吸光度和酰胺基Ⅱ吸光度之比分别在8,4,8.6,6,6 h达到最大值:1.7286,1.6190,1.6690,1.6460,1.6103,1.5905。结论:壳聚糖及其衍生物可使角质层含水量增加,可能是其具备透皮吸收促进作用的原因之一。
OBJECTIVE: To investigate the effect of chitosan (CS) and its derivatives on water content of stratum corneum in mice by attenuated total reflectance Fourier transform infrared spectroscopy (FTIR), and to explore its mechanism of action as a transdermal absorption enhancer. Methods: N-trimethyl chitosan (TMC) and N-carboxymethyl chitosan (MCC) were synthesized with different degrees of substitution. The stratum corneum of mice was extracted by enzymatic digestion and the cells were treated with 2.5% TMC, After treatment, ATR-FTIR analysis was performed to calculate the ratio of amido group I absorbance and amido group II absorbance. Results: After treated with TMC20, TMC40, TMC60, MCC, CS and H_2O (as control), the ratio of amidoⅠ absorbance to amido Ⅱ absorbance reached the maximum at 8,4,8.6,6 and 6 h respectively: 1.7286 , 1.6190, 1.6690, 1.6460, 1.6103, 1.5905. Conclusion: Chitosan and its derivatives can increase the moisture content of the stratum corneum, which may be one of the reasons for its role in promoting transdermal absorption.