估价频率有限稀释分析的最简单和最广泛的应用是测定含量很少的细胞的频率.尤其在研究中枢和外周免疫器官的淋巴细胞及其亚群的个体发生,和测定抗原或耐受原刺激后抗原特异细胞的数量变化方面,具有重要价值.例如,淋巴细胞在体外接触耐受原后,使能被激活而克隆增殖的抗原特异细胞的频率降低.耐受原的剂量越大,其频率降低越显著.任何没有产生耐受的细胞则能形成正常大小的克隆.换句话说,这种体外耐受的等级剂量效应(graded dose effect),反映耐受原的某一浓度灭活一个前体B细胞的能力.如果一个B细胞不被灭活,那它就可以进一步形成一个正常大小的克隆,其增生的潜力不受影响.在许多B细胞耐受系统的隔开分析(partition analysis)都不能检查出应答细胞的存在.这一点支持耐受的克隆 Valuation Frequency The simplest and most widely used method for limited dilution analysis is the determination of the frequency of cells with low levels of activity, especially in the study of individual occurrence of lymphocytes and their subpopulations in central and peripheral immune organs and in the determination of antigen or tolerance to primary irritation For example, the frequency of antigen-specific cells that can be activated to clonal proliferation is reduced by lymphocytes contacting tolerogenic in vitro, the greater the dose of tolerogenic, the greater the frequency The more pronounced the decrease, any non-tolerant cells are able to form normal-sized clones.In other words, this graded dose effect of in vitro tolerance reflects a certain concentration of tolerogenic inactivation of one The ability of a B cell to function as a B cell If a B cell is not inactivated then it can further form a normal sized clone with no potential for its proliferative potential In many B cell tolerant systems the partition analysis Can not detect the presence of responding cells, which supports tolerant clones