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目的探讨不同浓度的胶质源性神经营养因子(GDNF)对运动神经元的诱向生长作用。方法应用运动神经元体外诱向生长模型,将不同浓度的GDNF置于该模型中形成稳定的浓度梯度,72 h细胞培养后,通过测量模型中运动神经元生长锥的诱向生长的角度及神经元芽突的长度来初步筛选对运动神经元诱向作用最佳的GDNF浓度。结果 GDNF 200 ng/mL组与GDNF 100 ng/mL组间无明显区别(P>0.05);GDNF 200 ng/mL组及GDNF100 ng/mL组与空白对照组及GDNF 50 ng/mL组间比较,前两组均明显优于后两组,差异均具有统计学意义(P<0.05);空白对照组与GDNF 50 ng/mL组间差异明显(P<0.05)。结论 GDNF对运动神经元有明显的诱向生长作用,研究中初步确立了体外GDNF对运动神经元细胞诱向作用的最佳浓度为100 ng/mL。
Objective To investigate the effects of different concentrations of glial cell line-derived neurotrophic factor (GDNF) on motor neuron growth. Methods GDNF with different concentrations was placed in the model to establish a stable concentration gradient. The cultured neurons were cultured for 72 h. After the growth of the motor neuron growth cone was observed in the model, The length of the buds of the buds was used to initially screen for the best concentrations of GDNF for motor neuron induction. Results There was no significant difference between GDNF 200 ng / mL group and GDNF 100 ng / mL group (P> 0.05). Compared with blank control group and GDNF 50 ng / mL group, GDNF 200 ng / mL group and GDNF 100 ng / The first two groups were significantly better than the latter two groups, the differences were statistically significant (P <0.05); blank control group and GDNF 50 ng / mL group difference was significantly (P <0.05). Conclusion GDNF can induce motor neuron growth obviously. The optimal concentration of GDNF for motor neuron in vitro is 100 ng / mL.