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针对离心式微流控生化分析芯片的结构特点,设计了一种基于吸光度检测技术的光探测系统。通过双波长检测方法降低了闪烁氙灯光强输出稳定性差对吸光度检测结果的影响,采用同步触发的定位方式实现了检测过程中芯片的精确定位,并分析了进样误差对吸光度检测结果的影响。实验结果显示,采用双波长检测方法时系统吸光度重复性为0.0994%CV(变异系数);同步触发定位误差极小,在该种定位方式下的吸光度重复性为0.1429%CV;样品与试剂的进样重复性小于2%CV,满足国家标准。该系统结构简单,易于集成,检测结果稳定可靠,在医学临床诊断领域具有重要的实用价值。
Aiming at the structural characteristics of the centrifugal microfluidic biochemical analysis chip, an optical detection system based on absorbance detection technology was designed. The dual-wavelength detection method is used to reduce the influence of poor light output stability of xenon lamp on the result of absorbance measurement. The synchronous positioning method is used to realize the precise positioning of the chip during the detection process. The effect of injection error on absorbance measurement results is also analyzed. The experimental results show that the repeatability of the system absorbance is 0.0994% CV (coefficient of variation) when the dual wavelength detection method is used, the positioning error of the synchronization trigger is extremely small, and the repeatability of absorbance under this kind of positioning mode is 0.1429% CV. Repetitive less than 2% CV, to meet the national standards. The system has the advantages of simple structure, easy integration and stable and reliable test results, and has important practical value in the field of medical clinical diagnosis.