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目的观察自发性高血压大鼠(spontaneouslyhypertensiverats,SHR)心肌细胞凋亡和Bax/Bcl-2蛋白的表达;同时观察血管紧张素Ⅱ-1型受体(angiotensinⅡ1typereceptor,AT1R)拮抗剂-厄贝沙坦对SHR大鼠心肌细胞凋亡和BcL-2/Bax蛋白表达的影响。方法24只16周龄SHR大鼠(上海市高血压研究所提供,医动字02-37-1号)分为SHR治疗组[沙坦30mg/(kg·d),20周]和SHR对照组,另选16周龄Wistar大鼠12只作为正常对照组。分别采用SP免疫组化法和末端脱氧核苷酸转移酶介导dUTP缺口末端标记法方法检测心肌细胞凋亡指数和BcL-2/Bax蛋白水平的表达,放射免疫法检测血浆和组织血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)。结果SHR治疗组与正常对照组比较收缩压明显增高[(243±13)和(151±11)mmHg,1mmHg=0.133kPa],左心室质量与体质量比(leftventricularmass/bodymass,LVW/BW)明显增加[(4.05±0.33)和(2.90±0.23)g/kg];血浆和心肌组织AngⅡ增高[血浆(31.8±5.4)和(22.2±18.5)ng;心肌(13.2±2.1)%和(8.3±1.2)%];心肌细胞凋亡指数明显增加[(4.75±0.70)%和(2.84±0.60)%];心肌细胞Bax蛋白的表达明显增强[(5.02±0.34)%和(2.85±0.29)%],Bcl-2/Bax比率明显降低(0.65±0.13和1.05±0.18),差异有显著性意义(P<0.05～0.01);而Bcl-2蛋白的表达[(3.2
Objective To observe the apoptosis of cardiomyocytes and the expression of Bax / Bcl-2 protein in spontaneously hypertypertensive rats (SHR) and to observe the effects of angiotensin Ⅱ type 1 receptor antagonist - irbesartan On cardiomyocyte apoptosis and BcL-2 / Bax protein expression in SHR rats. Methods Twenty-six SHR rats (shang 30mg / (kg · d), 20 weeks) and SHR control The other 12 Wistar rats were selected as normal control group. The expression of Bcl-2 / Bax protein and cardiomyocyte apoptosis index were detected by SP immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling, respectively. The plasma and tissue angiotensin Ⅱ (angiotensin Ⅱ, Ang Ⅱ). Results Compared with the normal control group, systolic blood pressure (SHR) in the SHR treatment group was significantly higher (243 ± 13 and 151 ± 11 mmHg, 1 mmHg = 0.133 kPa), and left ventricular mass to body mass (LVW / BW) (31.5 ± 0.33) and (2.90 ± 0.23) g / kg] in plasma and myocardium [ 1.2%). The apoptotic index of cardiomyocytes increased significantly ([4.75 ± 0.70]% vs (2.84 ± 0.60)%], and the expression of Bax protein increased significantly in cardiomyocytes [(5.02 ± 0.34)% and ], The ratio of Bcl-2 / Bax was significantly lower (0.65 ± 0.13 and 1.05 ± 0.18), the difference was significant (P <0.05-0.01); while the expression of Bcl-