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Aim: To investigate possible changes in relative mitochondrial DNA (mtDNA) content in patients with non-arteritic anterior ischaemic optic neuropathy (NAION). Methods: 19 patients withNAIONwere compared to 32 controls matched for age, sex distribution, and ethnicity. DNA was extracted from leucocytes and competitive multiplex polymerase chain reaction was carried out with two primer pairs (one pair for mtDNA ND1 gene and the other pair for βactin nuclear gene) in the presence of a fluorescent dye. Results: The mean relative mtDNA content in controls (0.93 (SD 0.11); 95%CI 0.89 to 0.97) was signifi cantly less than in NAION patients (2.40 (1.05); 95%CI 1.90 to 2.91; p < 0.001). Relative mtDNA content was negatively correlated with Snellen visual acuity (Spearman’s rho; r=-0.37; p=0.022). Conclusion: Increased relative mtDNA content in NAION patients may imply a response to oxidative stress, possibly in part because of mitochondrial respiratory chain defects. Significantly more non-synonymous mtDNA nucleotide changes, significantly increased relative mtDNA content, and a significant association between relative mtDNA content and visual acuity all imply that mitochondrial abnormalities may be a risk factor for NAION.
Aim: To investigate possible changes in relative mitochondrial DNA (mtDNA) content in patients with non-arteritic anterior ischaemic optic neuropathy (NAION). Methods: 19 patients with NAIONwere compared to 32 controls matched for age, sex distribution, and ethnicity. DNA was extracted from leucocytes and competitive multiplex polymerase chain reaction was carried out with two primer pairs (one pair for mtDNA ND1 gene and the other pair for βactin nuclear gene) in the presence of a fluorescent dye. Results: The mean relative mtDNA content in controls (0.93 (SD 0.11); 95% CI 0.89 to 0.97) was signifi cantly less than in NAION patients (2.40 (1.05); 95% CI 1.90 to 2.91; p <0.001). Relative mtDNA content was negatively correlated with Snellen visual acuity Significantly more non-syno nymous mtDNA nucleotide changes, significantly increased relative mtDNA content, and a significant association between relative mtDNA content and visual acuity all imply that mitochondrial abnormalities may be a risk factor for NAION.