论文部分内容阅读
目的:观察核桃叶提取液对四氯化碳(CCl_4)诱发小鼠急性肝损伤的保护作用。方法:取昆明种小鼠50只,随机均分为正常对照组、模型对照组(给予生理盐水灌胃)及核桃叶低、中、高浓度组(分别给予0.1、0.2、0.4 g/ml的核桃叶提取液灌胃),灌胃量按10 ml/kg体重计算,每天灌胃1次,共14 d。在末次灌胃1 h对正常对照组(给予生理盐水)、模型对照组和核桃叶低、中、高浓度组(给予0.1%CCl_4)腹腔注射,注射量按10 ml/kg的体重计算,24 h后检测小鼠肝匀浆总蛋白(TP)浓度和谷丙转氨酶(GPT)活性、血清GPT活性。结果:肝匀浆TP浓度和GPT活性在各组间两两比较差异均无统计学意义(P>0.05),模型对照组和核桃叶低浓度组的血清GPT活性明显高于正常对照组,差异均有统计学意义(P<0.01或P<0.05);核桃叶中浓度组和高浓度组的血清GPT活性与正常对照组比较差异均无统计学意义(P>0.05);核桃叶低、中、高浓度组和模型对照组的组间两两比较差异均无统计学意义(P>0.05);随着核桃叶提取液浓度的增加血清GPT活性趋向正常对照组。结论:血清GPT活性升高是肝细胞受损的早期敏感指标,核桃叶提取液达到一定浓度具有一定预防自由基破坏肝细胞的功能。
Objective: To observe the protective effect of walnut leaf extract on acute liver injury induced by carbon tetrachloride (CCl_4) in mice. Methods: Fifty Kunming mice were randomly divided into normal control group, model control group (intragastric administration of normal saline) and low, medium and high concentration of walnut leaves (0.1, 0.2, 0.4 g / ml Walnut leaf extract gavage), gavage according to 10 ml / kg body weight, gavage 1 day for a total of 14 d. Rats in the normal control group (given normal saline), model control group and low, medium and high concentration of walnut leaves (given 0.1% CCl 4) were injected intraperitoneally at the dose of 10 ml / kg at 1 h after the last intragastric administration. h after the test, the total liver protein (TP) concentration, the alanine aminotransferase (GPT) activity and the serum GPT activity were detected. Results: There was no significant difference in the concentrations of TP and GPT in each group between the two groups (P> 0.05). The serum GPT activity in model control group and low-concentration group was significantly higher than that in normal control group (P <0.01 or P <0.05). There was no significant difference in serum GPT activity between the control group and the control group (P> 0.05) (P> 0.05). With the increase of concentration of walnut leaf extract, serum GPT activity tended to the normal control group. CONCLUSION: Elevated serum GPT activity is an early sensitive indicator of liver cell damage. When the extract of walnut leaf reaches a certain concentration, it can prevent the free radical from damaging the liver cells.