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研究茶多酚 (TP)对顺铂 (DDP)所致肾毒性作用的保护作用。方法 小鼠随机分为对照组、DDP组、DDP+硫代硫酸钠(STS)组、DDP+高剂量 TP组、顺铂 +低剂量 TP组 ,观察血清中尿素氮 (BUN)、肌酐 (Cr)变化情况 ,测定肾组织匀浆中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH-Px)的活性及肾脏电镜超微结构的变化。结果 DDP组的血清中 BUN上升 ,与阴性对照组比较 ,差异有显著性 ;加入高剂量 TP和 STS以后 ,血清中 BUN值与 DDP组比较 ,差异有显著性。给予 DDP后 SOD、GSH-Px活性降低与生理盐水组比较 ,差异有显著性 ;DDP+ STS组、DDP+高剂量 TP组与 DDP组比较 ,差异有显著性。电镜结果表明 :加入高剂量 TP组与 DDP组比较 ,肾脏线粒体、刷状缘微绒毛损伤有明显减轻。结论 TP在一定程度上能预防 DDP所致的肾毒性。
To study the protective effect of tea polyphenols (TP) on nephrotoxicity induced by cisplatin (DDP). Methods The mice were randomly divided into control group, DDP group, DDP + sodium thiosulfate (STS) group, DDP + high dose TP group and cisplatin + low dose TP group. The levels of serum urea nitrogen (BUN) and creatinine The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in renal homogenate and the ultrastructure of renal tissue were measured. Results The serum levels of BUN in DDP group increased significantly compared with that in the negative control group. After adding high-dose TP and STS, the serum BUN value was significantly different from that in DDP group. Compared with normal saline group, the decrease of SOD and GSH-Px activity after DDP administration was significant. The difference was significant between DDP + STS group, DDP + high-dose TP group and DDP group. Electron microscopy results showed that the damage of mitochondria and brush border microvilli in kidneys was significantly reduced compared with DDP group. Conclusion TP can prevent nephrotoxicity induced by DDP to a certain extent.