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目的 动态观察硫喷妥钠对大鼠不同脑区NOS活性、NO的影响 ,探讨硫喷妥钠的全麻作用机制。方法 ♂SD大鼠 40只 ,按麻醉不同时期随机分为 5组。分别在腹腔注射 (ip) 10ml·kg-1生理盐水 5min后 (对照组 ) ,ip硫喷妥钠 30mg·kg-12min后、翻正反射消失前 (诱导期组 ) ,翻正反射消失后 5min(麻醉期组 ) ,翻正反射刚恢复、尚未完全清醒时 (恢复期组 ) ,动物完全清醒后 (清醒期组 ) ,断头取脑。用还原酶法测定大脑皮层、脑干的NOS活性和NO。结果 与对照组比较 ,硫喷妥钠 30mg·kg-1使皮层、脑干的NOS活性在诱导期即降低 (P <0 0 5 ,P <0 0 1) ;麻醉期进一步降低 ;恢复期开始恢复 ,但仍低于对照组 (P <0 0 5 ) ;清醒期基本恢复到对照水平。皮层、脑干的NO在诱导期即降低(P <0 0 5 ,P <0 0 1) ;麻醉期进一步降低 ;恢复期开始恢复 ,但仍低于对照组 (P <0 0 5 ) ;清醒期基本恢复到对照水平。结论 硫喷妥钠 30mg·kg-1能明显影响大鼠脑NOS活性和NO ,表明NO可能在硫喷妥钠的全麻分子学机制中发挥重要作用。
Objective To observe the effect of thiopental on NOS activity and NO in different brain regions of rats and to explore the general anesthetic mechanism of thiopental sodium. Methods 40 male SD rats were randomly divided into 5 groups according to different periods of anesthesia. The rats in each group were injected intraperitoneally (ip) 10ml · kg-1 normal saline for 5min (control group), ip thiopental 30mg · kg-12min before the disappearance of right reflex (induction group), disappearance of right reflex after 5min (Anesthesia group), righting reflex had just recovered, not yet fully awake (convalescent group), animals completely awake (awake group), decapitated brain. NOS activity and NO in cerebral cortex and brainstem were measured by reductase method. Results Compared with the control group, NOS activity in cortex and brainstem decreased at 30 mg · kg-1 of thiopental (P <0.05, P <0.01), and decreased at anesthesia; Recovery, but still lower than the control group (P <0 05); awake recovery to the control level. NO in the cortex and brainstem decreased at the induction stage (P <0.05, P <0.01), decreased further during anesthesia, recovered at recovery, but still lower than that in the control group (P <0.05) Period basically returned to the control level. Conclusion Thiopental 30mg · kg-1 can significantly affect NOS activity and NO in rat brain, indicating that NO may play an important role in the molecular mechanism of general anesthesia with thiopental.