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目的:建立苦豆子中总黄酮含量测定方法,并对苦豆子各营养器官中总黄酮含量进行测定。方法:以芦丁为对照品,采用紫外分光光度法于510nm波长处测定苦豆子中总黄酮的含量。结果:芦丁在0.1834～0.9170g/L范围内线性关系良好(n=5),相关系数r=0.9994,平均回收率为99.57%。结论:该方法检测结果准确,操作简便,重复性好,可作为苦豆子药材中总黄酮含量检测的方法。
Objective: To establish a method for the determination of total flavonoids in Sophora alopecuroides and to determine the content of total flavonoids in Sophora alopecuroides vegetative organs. Methods: Rutin was used as reference substance and the content of total flavonoids in Sophora alopecuroides was determined by ultraviolet spectrophotometry at 510nm wavelength. Results: The linearity of rutin in the range of 0.1834 ~ 0.9170g / L was good (n = 5), the correlation coefficient was 0.9994, the average recovery was 99.57%. Conclusion: The method is accurate, simple, reproducible and can be used as a method for the determination of total flavonoids in Sophora alopecuroides.