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通过电镜和~(125)Ⅰ—udR标记肿瘤靶细胞DNA释放试验,对腹腔或皮下注射SpA或SpA菌体后小鼠腹腔巨噬细胞的超微结构、及其对靶细胞的细胞毒效应进行了动态观察。结果首次证明,小鼠腹腔注射SpA菌体后第5、14d,其腹腔巨噬细胞体积明显增大,可达对照组的2—3倍;皱褶明显增多,有众多的伪足和指状突起,外形极不规则,形似海星;胞质内细胞器明显增多,尤以粗面内质网和溶酶体为显著,胞质内充满吞噬体。实验进一步证明,小鼠腹腔注射SpA或SpA菌体后第5d,其腹腔巨噬细胞杀伤效应(细胞毒分别为42.0±7.8％和46.3±11.7％)比对照组(细胞毒为32.0±13.8％)明显增强,至第7d,细胞毒效应达峰值水平(分别为50.4±17.3％和56.9±13.4％)。根据实验结果,对SpA增强巨噬细胞杀伤效应的可能机理进行了讨论。
The ultrastructure of mouse peritoneal macrophages and their cytotoxic effects on target cells after intraperitoneal or subcutaneous injection of SpA or SpA cells were investigated by electron microscopy and ~(125)I-udR labeling of tumor target cell DNA release assays. Dynamic observation. The results demonstrated for the first time that the volume of peritoneal macrophages on the 5th and 14th days after intraperitoneal injection of SpA cells increased significantly, reaching 2-3 times the control group; wrinkles increased significantly, and numerous pseudopodia and fingers were found. Protuberant, very irregular shape, the shape of starfish; cell organelles increased significantly, especially in the rough endoplasmic reticulum and lysosomes, the cytoplasm is full of phagosomes. The experiment further proves that the cytotoxicity of the peritoneal macrophages (40% cytotoxicity and 46.3±11.7% respectively) is higher than that of the control group (cytotoxicity 32.0±13.8%) at the 5th day after intraperitoneal injection of SpA or SpA cells. Significantly increased, to the 7th day, the cytotoxic effect peaked (50.4±17.3% and 56.9±13.4%, respectively). Based on the experimental results, the possible mechanism of SpA-induced macrophage killing effect was discussed.