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近年来,我室利用多种动物模型,在整体或离体实验条件下,证明酪氨酸有抑制hCG 致孕酮生成的作用。推测,酪氨酸可能作为卵巢内调节因子之一,对卵巢孕激素合成过程发挥局部反馈调节作用。本文的目的是,利用同位素示踪技术,观察小鼠下丘脑、垂体、卵巢对~3H 酪氨酸的摄取情况,着重探讨影响卵巢摄取酪氨酸的一些因素。材料与方法本实验用26-30克雌性未育小鼠,饲养于20℃的室温中,光照14小时,黑暗10小时,水食任摄。每日作阴道涂片,选间情期(D_2)小鼠,实验小鼠由尾静脉注射~3H 酪氨酸(放射比强
In recent years, our laboratory has used a variety of animal models to demonstrate that tyrosine inhibits the production of progesterone by hCG, either in whole or in vitro experiments. Speculated that tyrosine may act as one of the ovarian regulators, progesterone synthesis of ovarian play a local feedback regulation. The purpose of this paper is to investigate the uptake of 3H tyrosine by the hypothalamus, pituitary and ovary in mice using isotope tracing technique and to explore some of the factors that affect tyrosine uptake in ovaries. Materials and Methods In this experiment, 26-30 g female mice were housed in room temperature at 20 ℃ for 14 hours and dark for 10 hours. Vaginal smear daily, the selection between the affectionate (D_2) mice, experimental mice injected from the tail vein ~ 3H tyrosine (strong radiation