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The autonomous Mutator(Mu)transposon in maize(Zea mays L.)lines 115F,330I,and 715D were crossed with inbred lines B73,Mo17,97108,and H9-21,M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the field.Next we statistically analyzed these candidate mutants.Under drought stress,we screened seedlings at 3-leaf stage by using the infrared thermography,and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines,which temperature were significant different with others.These candidate mutants were primarily analyzed by infrared thermography,chlorophyll fluorescence,leaf water losing,photosynthetic characteristics,content of soluble sugars,soluble protein,and proline assay.The selected mutants were cloned by MuTAIL–PCR methods.Here we provide the better genetic materials for research on maize breeding for drought tolerance.
The autonomous Mutator (Mu) transposon in maize (Zea mays L.) lines 115F, 330I, and 715D were crossed with inbred lines B73, Mo17, 97108, and H9-21, M1were self-pollinated to establish the Mu insertion-mutagenized M2seeds pool and The M2plants encompassed a large amount of biological variation relevant to improving agronomy traits by observed in the field .Next weathered analyzed these candidate mutants .Under drought stress, we screened seedlings at 3-leaf stage by using the infrared thermography, and successfully got 108 droughtinsensitive and 121 drought-sensitive candidate mutants from more than 38,000 lines, where temperature were significant different with others. These candidate mutants were analyzed by infrared thermography, chlorophyll fluorescence, leaf water losing, photosynthetic characteristics, content of soluble sugars, soluble protein, and proline assay. selected mutants were cloned by MuTAIL-PCR methods. Here we provide the better genetic materials for research on maize breeding for drought tolerance.