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目的评价低温等离子体灭活水中细菌的效果,探讨等离子体效应对革兰阴性菌和革兰阳性菌的灭活机制。方法观察低温等离子体与水中初始浓度为(9.60±1.23)×106 cfu/ml的大肠杆菌和初始浓度为(5.71±3.14)×107 cfu/ml的金黄色葡萄球菌分别反应10、30、60、120 s后的灭活效果,采用透射电镜(TEM)对细菌超微结构的变化进行分析。结果大肠杆菌在灭菌30 s时灭活率达6.98 log,金黄色葡萄球菌在灭菌10 s时灭活率达7.76 log,水中大肠杆菌和金黄色葡萄球菌全部被灭活。大肠杆菌(革兰阴性菌)出现细胞变形和胞质整体不均匀;而金黄色葡萄球菌(革兰阳性菌)则出现体积膨胀和核心消失,菌体近似空壳。结论低温等离子体在短时间内对水中大肠杆菌和金黄色葡萄球菌具有明显灭活作用。
Objective To evaluate the effect of low temperature plasma inactivation of bacteria in water and to explore the mechanism of plasma inactivation against Gram-negative and Gram-positive bacteria. Methods Low temperature plasma was reacted with Escherichia coli (9.60 ± 1.23) × 106 cfu / ml and (5.71 ± 3.14) × 107 cfu / ml initial concentration in water to react respectively with 10, 30, After 120 s inactivation, the changes of bacterial ultrastructure were analyzed by transmission electron microscopy (TEM). Results The inactivation rate of Escherichia coli reached 6.98 log after 30 s, while the inactivation rate of Staphylococcus aureus reached 7.76 log after 10 s, and all of Escherichia coli and Staphylococcus aureus were inactivated in water. Escherichia coli (Gram-negative bacteria) cell deformation and cytoplasmic overall heterogeneity; and Staphylococcus aureus (Gram-positive bacteria) is the volume expansion and core disappear, the cell shell is almost empty. Conclusion Low-temperature plasma has obvious inactivation of Escherichia coli and Staphylococcus aureus in water in a short time.