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[目的]研究草莓炭疽病(Colletotrichum fragariae)的生物防治方法。[方法]采用菌丝生长抑制法测定枯草芽孢杆菌(Bacillus subtilis)(BS)和蛇床子素(SC)对草莓炭疽病菌的室内抑菌活性,并进行田间防治试验。[结果]室内测定结果表明,BS和SC对草莓炭疽病菌菌丝生长都有极强的抑菌活性,其EC50值分别为0.007 5和1.063 0 ml/L。田间防治结果显示,无论在避雨或露天条件下,BS(用量600~750 g/hm2)和25%咪鲜胺EC(用量600 ml/hm2),在防治3次后7和14天的防治效果均达76%以上,但两药剂与SC(用量1 800~2 700 ml/hm2)的田间防治效果间有极显著差异(P<0.01)。[结论]枯草芽孢杆菌可以用于草莓炭疽病的防控。
[Objective] The research aimed to study the biological control methods of Colletotrichum fragariae. [Method] The mycelium growth inhibition method was used to determine the antibacterial activity of Bacillus subtilis (BS) and osthole (SC) to strawberry strawberry anthracnose and the field experiments were conducted. [Result] The indoor test results showed that BS and SC had extremely strong antibacterial activity against mycelium growth of strawberry anthracnose, the EC50 values were 0.007 5 and 1.063 0 ml / L, respectively. The results of field control showed that under the condition of shelter from rain or open air, BS (dosage 600 ~ 750 g / hm2) and 25% prochloraz EC (dosage 600 ml / hm2) The results showed that there was a significant difference (P <0.01) in the field control effect between the two agents and SC (the dosage of 1 800-2 700 ml / hm2). [Conclusion] Bacillus subtilis could be used for the prevention and control of strawberry anthracnose.