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将编码人白细胞介素-4(IL-4)的cDNA插入苜蓿银纹夜蛾单核多角病毒(Autogranhacalifor-nicanuclearpolyhedrosisvirus,AcNPV)的多角体蛋白基因启动子下游,构建表达IL-4的重组病毒。感染该重组病毒的细胞可表达较高水平的IL-4多肽。所表达的重组IL-4具有较高的生物学活性,分子量为16KDa。
A cDNA encoding human interleukin-4 (IL-4) was inserted downstream of the polyhedrin gene promoter of Autogranhacalifor-nicanucleolyticrosis virus (AcNPV) to construct a recombinant virus expressing IL-4. Cells infected with the recombinant virus can express higher levels of IL-4 polypeptide. The expressed recombinant IL-4 has higher biological activity, molecular weight of 16KDa.