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光(温)敏核雄性不育水稻(Photoperiod/temperature-sensitivegenicmale-sterilerice,简称P/TGMR)89025和培矮64s在秋季从不育阶段经半不育阶段向可育阶段转变进程中,对其花粉母细胞形成期(Ⅴ)、花粉母细胞减数分裂期(Ⅵ)的幼穗和成熟花药(Ⅷ)中过氧化物酶(Peroxidse,简称POD)、超氧物歧化酶(Superoxidedismutase,简称SOD).和过氧化氢酶(Catalase,简称CAT)活性测定表明,幼穗和花药相同发育时期的酶活性随正常花粉的比率升高而增加,但以成熟花药中增加最多.并伴有丙二醛(Malonaldehyde,简称MDA)含量减少.表明自由基代谢的变化可能与光(温)敏核雄性不育水稻花粉败育有关,且自由基代谢变化在幼穗发育中期即已开始.
Photoperiod / temperature-sensitivegenicmale-sterilerice (P / TGMR) 89025 and Pei’ai 64s were transformed from sterile stage to fertile stage in autumn. (Ⅴ) of pollen mother cells, peroxidase (POD) and superoxide dismutase (SOD) in the young spikes and mature anthers (Ⅷ) of pollen mother cell meiosis stage (Ⅵ) ). And catalase (CAT) activity showed that the activity of young ear and anther at the same development stage increased with the increase of normal pollen rate, but increased most in mature anthers. Accompanied by reduced malonaldehyde (MDA) content. The results indicated that the change of free radical metabolism might be related to the pollen abortion of the male sterile rice with photo (thermo) sensitivity and that the change of free radical metabolism started at the mid-stage of panicle development.