本文对用MTT颜色反应法测淋巴细胞的转化做了一定的改良,细胞先在含小牛血清培养液中培养,在加入MTT 试剂前更换到不含小牛血清的培养液中,避免了沉淀形成影响比色,获得满意结果。我们用改良的颜色反应法对正丁基缩水甘油醚对淋巴细胞转化的影响进行了研究,发现正丁基缩水甘油醚可抑制淋巴细胞转化。 In this paper, using MTT color reaction method to measure the conversion of lymphocytes made some improvements, the cells cultured in medium containing bovine serum before adding MTT reagent to the medium without bovine serum to avoid precipitation The formation of colorimetric effects, to obtain satisfactory results. We studied the effect of n-butyl glycidyl ether on lymphocyte transformation using a modified color reaction and found that n-butyl glycidyl ether inhibited lymphocyte transformation.