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The determination method of L-phenylalanine(LPA) by fluorescence quenching was developed.The assay was based on the combination of the cucurbit[7]uril(CB[7]) with palmatine hydrochloride(PAL) reaction.In the presence of CB[7],the fluorescence of PAL was quenched by LPA which can be employed to detect LPA.Under the optimal conditions,a linear range 3.63×10~(-8)- 9.68×10~(-6)mol/L and a detection limit 1.27×10~(-8)mol/L of LPA were obtained.The relative standard deviation(R.S.D) was 1.8%obtained from a series of 11 standards each containing 6.05×10~(-6) mol/L of LPA.This paper also discusses the mechanism of fluorescence indicator probe.
The determination method of L-phenylalanine (LPA) by fluorescence quenching was developed. The assay was based on the combination of the cucurbit [7] uril (CB [7]) with palmatine hydrochloride 7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA.Under the optimal conditions, a linear range of 3.63 × 10 -8 -8.68 × 10 -6 mol / L and a detection limit 1.27 × 10 -8 mol / L of LPA was obtained. The relative standard deviation (RSD) was 1.8% obtained from a series of 11 standards each containing 6.05 × 10 -6 mol / L of LPA. This paper also discusses the mechanism of fluorescence indicator probe.