用接种日本血吸虫(Sj)尾蚴8周的BALB/c小鼠脾细胞与P_3-X63-Ag8-653骨髓瘤细胞进行融合。3周后,用ELISA检测该杂交瘤细胞分泌的抗Sj虫卵抗体。并用SRBC作间接放射免疫位点试验,检测其分泌的嗜异抗体。大约10～60％孔的杂交瘤细胞株能生长,其中20～30％能分泌抗体。将产 The BALB / c mouse spleen cells that were inoculated with cercariae of Schistosoma japonicum (Sj) for 8 weeks were fused with P_3-X63-Ag8-653 myeloma cells. After 3 weeks, the anti-Sj antibody secreted by the hybridoma cells was detected by ELISA. And with SRBC indirect radioimmunoassay test, detection of the secretion of the heterophile. About 10 to 60% of the wells can grow hybridoma cell lines, of which 20 to 30% can secrete antibodies. Will produce