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根据国际上以往的研究,许多百合种间远缘杂交时不能受精,即使受精后其种胚也不能正常发育。胚不能正常发育的主要原因是胚与胚乳不亲和或胚乳败育(1、7、9),而各种百合杂交不亲和的机制主要由花柱控制(1、10)。因此,试验中我们采用了切割花柱和幼胚离体培养法,来进行百合种间远缘杂交。
According to the international research in the past, many hybrids of Lilium can not be fertilized when their hybridization is far. Even after fertilization, their embryos can not develop normally. The main reason for the inability of embryos to develop normally is the incompatibility between embryos and endosperm (1, 7, 9), while the mechanism of incompatibility of various lilies is mainly controlled by styles (1, 10). Therefore, in the experiment, we used cut style and immature embryos in vitro culture method to carry out distant hybridization of lilies.