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目的:观察红花注射液对内毒素性急性肝损伤大鼠的干预作用及可能的机制。方法:将清洁级雄性SD大鼠80只随机分为模型组、红花注射液干预组(红花组)、还原型谷胱甘肽干预组(TAD组)及空白对照组,模型组时相点设为6h、24h、48h,红花注射液干预组、还原型谷胱甘肽干预组(TAD组)时相点同模型组;每时相点10只动物;空白对照组10只动物。模型组以腹腔注射内毒素脂多糖(LPS)+D-氨基半乳糖胺(D-GalN)诱导建立大鼠急性肝损伤模型;红花组、TAD组于建模前0.5h分别以红花注射液、还原型谷胱甘肽尾静脉注射;模型组、红花组、TAD组分别于建模后6h、24h、48h及空白对照组于6h时取腹腔静脉血和肝组织,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),苏木素-伊红(HE)染色观察肝组织病理学变化,免疫组织化学法检测肝组织Bax、Bcl-2蛋白表达并计算其比值(Bax/Bcl-2)。结果:与空白对照组比较,模型组各时间点大鼠血清ALT、AST水平显著升高(均P<0.01);肝组织Bax、Bcl-2表达及其比值明显上升(均P<0.01)。与模型组相比,红花组、TAD组各时间点大鼠肝组织病理损伤程度明显改善;血清ALT、AST水平不同程度地下降(P<0.05或P<0.01);肝组织Bax、Bcl-2表达及Bax/Bcl-2下降(均P<0.01)。红花组与TAD组间比较差异均无统计学意义(P>0.05)。结论:红花注射液对内毒素性急性肝损伤有较好的保护作用,其机制可能与下调肝组织Bax、Bcl-2表达及其比值,抑制肝组织细胞过度凋亡相关。
Objective: To observe the effects of safflower injection on endotoxin-induced acute liver injury in rats and its possible mechanism. Methods: Eighty clean male SD rats were randomly divided into model group, safflower injection intervention group (safflower group), reduced glutathione intervention group (TAD group) and blank control group. The model group phase Point set to 6h, 24h, 48h, safflower injection intervention group, reduced glutathione intervention group (TAD group) at the same time point with the model group; each time point 10 animals; control group of 10 animals. The acute liver injury model was established by intraperitoneal injection of lipopolysaccharide (LPS) and D-galactosamine (D-GalN) in the model group. In the safflower and TAD groups, Liquid and reduced glutathione tail vein injection; model group, safflower group, TAD group at 6h, 24h, 48h after modeling and blank control group at 6h to take celiac venous blood and liver tissue, Pathological changes of liver were observed by ALT, AST and HE staining. The expression of Bax and Bcl-2 in liver tissue were detected by immunohistochemistry and the ratio of Bax / Bcl-2). Results: Compared with the blank control group, the levels of serum ALT and AST were significantly increased in the model group at each time point (all P <0.01), and the expressions of Bax and Bcl-2 in the liver tissue were significantly increased (all P <0.01). Compared with the model group, the pathological changes of liver tissue of rats in safflower group and TAD group were significantly improved at each time point; serum ALT and AST levels were decreased to different extents (P <0.05 or P <0.01); Bax and Bcl- 2 expression and decreased Bax / Bcl-2 (all P <0.01). There was no significant difference between safflower group and TAD group (P> 0.05). CONCLUSION: Safflower injection has a good protective effect on endotoxin-induced acute liver injury. The mechanism may be related to the down-regulation of Bax and Bcl-2 expression and its ratio in liver tissue and the inhibition of excessive apoptosis in liver cells.