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目的探讨白细胞介素4(Interleukin 4,IL-4)基因多态性和幽门螺杆菌(Helicobacter pylori,H.pylori)感染与广西柳州地区胃癌易感性的关系。方法采用前瞻性研究,用多聚酶链式反应(PCR)和半巢式聚合酶链式反应(Semi-nested PCR)对112例胃癌患者及238例慢性胃炎患者H.pylori的尿素酶B亚单位(UreB)基因和IL-4-588基因多态性进行分析。结果胃癌组H.pylori阳性率为59.8%,高于对照组的47.5%(P<0.05)。两组患者IL-4-588各基因型(T/T型、C/T和C/C型)分布频率差异无统计学意义(χ2=0.627,P=0.73);胃癌组含等位基因C、T者分别为的40例(17.9%)、184例(82.1%),其分布频率与对照组的73例(15.3%)、403例(84.7%)差异无统计学意义(χ2=0.715,P=0.398)。结论 IL-4-588基因多态性与H.pylori感染之间不存在交互作用,且与广西柳州地区胃癌易感性无关。
Objective To investigate the relationship between Interleukin 4 (IL-4) gene polymorphism and Helicobacter pylori (H.pylori) infection and susceptibility to gastric cancer in Liuzhou of Guangxi. Methods A prospective study was carried out to investigate the relationship between urease B subunit of H.pylori in 112 gastric cancer patients and 238 chronic gastritis patients by polymerase chain reaction (PCR) and semi-nested polymerase chain reaction (PCR) UreB) gene and IL-4-588 gene polymorphism were analyzed. Results The positive rate of H.pylori in gastric cancer group was 59.8%, which was higher than that in control group (47.5%, P <0.05). There was no significant difference in the frequencies of IL-4-588 genotypes (T / T, C / T and C / C genotypes) between the two groups (χ2 = 0.627, P = 0.73) (17.9%) and 184 cases (82.1%) of T patients, respectively. There was no significant difference in the distribution frequency between the control group and the control group (73.3% vs 403.7%, 84.7% P = 0.398). Conclusion There is no interaction between IL-4-588 gene polymorphism and H.pylori infection, and it has no relationship with susceptibility to gastric cancer in Liuzhou, Guangxi.