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为了探讨甜菜耐盐机理、不同耐盐等级甜菜盐耐性机制差异,对40份甜菜品种(系)用300mmol·L-1NaCl胁迫处理,通过芽期相对盐害率和苗期盐害指数确定不同品种耐盐等级,将不同耐盐等级材料进行NaCl浓度梯度(150、300mmol·L-1)处理,测定植株鲜重、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和叶片质膜透性。试验结果如下:40份甜菜材料经过芽期和苗期耐盐性筛选最终确定耐盐等级极强的品种(系)1份(‘KWS0143’),耐盐等级强的品种(系)6份(‘ACERO’等),耐盐等级中等的品种(系)14份(‘HI0183’等),耐盐等级弱的品种(系)5份(‘OVITO’等);在同一盐胁迫时间下,不同耐盐等级材料的植株鲜重随盐浓度的增加呈下降趋势,品种‘KWS0143’和‘ACERO’变化幅度较小,品种‘OVATIO’下降幅度最大;不同耐盐等级材料随盐浓度增加和胁迫时间延长超氧化物歧化酶(SOD)活性呈现先上升而后下降的趋势,耐盐性极强的品种酶活性显著高于其他耐盐等级材料;不同耐盐等级材料随盐浓度增加和胁迫时间延长丙二醛(MDA)含量和叶片质膜透性均呈上升趋势,耐盐等级弱的品种(系)明显高于其他各个材料。
In order to investigate the mechanism of salt tolerance in sugar beet and the mechanism of salt tolerance in different salt tolerance grades, 40 sugar beet varieties (lines) were treated with 300 mmol·L-1 NaCl stress. The relative salt damage rate and salt damage index The salt tolerance of different salt tolerance grades was studied by NaCl concentration gradient (150, 300 mmol·L-1). The fresh weight, the activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) Membrane permeability. The test results are as follows: Forty sugarbeet materials were screened at salt-tolerant stage and seedling stage to determine 1 (KWS0143 ’), 6 ( ’ACERO’, etc.), 14 (’HI0183’ etc.) varieties with moderate salt tolerance, 5 (’OVITO’ etc.) varieties with weak salt tolerance, and different under the same salt stress time The fresh weight of the plants with salt tolerance decreased with the increase of salt concentration. The varieties ’KWS0143’ and ’ACERO’ showed less variation and the variety ’OVATIO’ had the largest decrease. The salt tolerance of different salt tolerant grades increased with the increase of salt concentration and stress time Prolonging the activity of superoxide dismutase (SOD) firstly and then descending, and the enzyme activity of the cultivars with extremely high salt tolerance was significantly higher than that of other salt-tolerant materials. The different salt-tolerant materials with increasing salt concentration and prolonging the stress duration MDA content and leaf plasma membrane permeability all showed an upward trend, and those with weak salt tolerance were significantly higher than those of other materials.