利用体内体外试验观察了中药“抗癌灵”对具有较强侵袭力的小鼠低分化肝癌（ＨＣａ－ｐ）的杀伤及抑制作用。抗癌灵２号及抗癌灵３号浓度达到０２ｇ／ｍｌ时，在体外对瘤细胞的杀伤率均达１００％；它们对移植瘤的抑制率分别为３３４％和３５８％，后者与对照组有显著差异（Ｐ＜００５）。抗癌灵３号对肝、心、肾均无明显毒性。结果说明抗癌灵３号对小鼠肝癌有一定治疗作用，且毒副作用小，可作为临床应用的参考。 The in vitro and in vivo experiments were performed to observe the killing and inhibiting effects of the traditional Chinese medicine “Anti-cancer spirit” on poorly-differentiated liver cancer (HCa-p) in mice with strong invasiveness. When the concentration of Kangailing 2 and Kangailing 3 reached 02g/ml, the killing rate of tumor cells in vitro reached 100%; their inhibitory rates to transplanted tumors were 334% and 358, respectively. %, the latter was significantly different from the control group (P < 0.05). Anti-cancer spirit No. 3 had no obvious toxicity to liver, heart and kidney. The results showed that Kangailing 3 has a certain therapeutic effect on liver cancer in mice, and has little side effects, which can be used as a reference for clinical application.