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AIM:To investigate the putative role of human papillomavirus(HPV) infection in the carcinogenesis of esophageal squamouscell carcinoma in China.METHODS:Twenty-three esophageal squamous cellcarcinoma samples and the distal normal epithelium from ShanxiProvince,and 25 more esophageal squamous cell carcinomasamples from Anyang city,two areas with a high incidence ofesophageal cancer in China,were detected for the existenceof HPV-16 DNA by PCR,mRNA in situ hybridization (ISH) andimmunohistochemistry (IHC) targeting HPV-16 E6 gene.RESULTS:There were approximately 64% (31/48) patientshaving HPV-16 DNA in tumor samples,among them nearly two-thirds (19/31) samples were detected with mRNA expression ofHPV-16 E6.However,in the normal esophageal epithelium fromcancer patients,the DNA and mRNA of HPV-16 were found withmuch less rate:34.7% (8/23) and 26.1% (6/23) respectively.In addition,at protein level detected by IHC assay,27.1% (13/48) tumor samples had virus oncoprotein E6 expression,whileonly one case of normal epithelium was found positive.CONCLUSION:HPV infection,especially type 16,should beconsidered as a risk factor for esophageal malignancies in China.
AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China. METHODS: Twenty-three esophageal squamous cellcarcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinomasamples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene. (31/48) patientshaving HPV-16 DNA in tumor samples, among them nearly two-thirds (19/31) samples were detected with mRNA expression of HPV-16 E6.However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7% (8/23) and 26.1% (6/23) respectively.In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, whileonly one case of normal epithelium was found positive. CONCLUSION: HPV infection, especially type 16, should beconsidered as a risk factor for esophageal malignancies in China.